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KMID : 0359319720120010071
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Korean Journal of Veterinary Research
1972 Volume.12 No. 1 p.71 ~ p.75
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Studies on Inactivated Combined Vaccine of Bovine Anthrax and Blackleg
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Abstract
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Due to the fact that an inactivated anthrax vaccine may show no or Bower immunogenicity and stability, a number of spore vaccines were exclusively used worldwide. In these studios non or less allergic strain of anthrax bacillus was selected and made a capsulated vegetative organisms.
Anthrax organisms of a virulent strain were cultivated on sodium bicarbonate medium with or without adding 1-alanine in which B. anthracis grew luxuriantly without forming spores. Inactivation of the organisms was carried out at 37℃ water bath for 3 days after the bacterial culture was mixed with formalin in a final concentration of two per cent. Aluminum hydroxide gel was added to the mixture of anthrax and blackleg bacterin. Guinea pigs were injected with the vaccine via subcutaneous or intramuscular route and challenged after three weeks, and the Possibilities of protection was tested.
Throughout the studies, the vaccines possibly protected the vaccinated guinea pigs more than 80per cent compared to that of the controls. This experimental results strongly suggest that the vaccine may possibly applicable to the prevention of bovine anthrax and blackleg.
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